Automated Micropipette Aspiration of Single Cells
نویسندگان
چکیده
منابع مشابه
Micropipette aspiration of living cells.
The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface. Such edges can be tracked in a light microscope to an accuracy of +/-25 nm and suction pressures as small as 0.1-0.2 pN/microm2 can be imposed on the cell. Both soft cells, such as neutrophils and red cel...
متن کاملMicrofluidic micropipette aspiration for measuring the deformability of single cells.
We present a microfluidic technique for measuring the deformability of single cells using the pressure required to deform such cells through micrometre-scale tapered constrictions. Our technique is equivalent to whole-cell micropipette aspiration, but involves considerably simpler operation, less specialized equipment, and less technical skill. Single cells are infused into a microfluidic chann...
متن کاملThe Application of Micropipette Aspiration in Molecular Mechanics of Single Cells.
Micropipette aspiration is arguably the most classical technique in mechanical measurements and manipulations of single cells. Despite its simplicity, micropipette aspiration has been applied to a variety of experimental systems that span different length scales to study cell mechanics, nanoscale molecular mechanisms in single cells, bleb growth, and nucleus dynamics, to name a few. Enabled by ...
متن کاملA Finite Element Study of Micropipette Aspiration of Single Cells: Effect of Compressibility
Micropipette aspiration (MA) technique has been widely used to measure the viscoelastic properties of different cell types. Cells experience nonlinear large deformations during the aspiration procedure. Neo-Hookean viscohyperelastic (NHVH) incompressible and compressible models were used to simulate the creep behavior of cells in MA, particularly accounting for the effect of compressibility, bu...
متن کاملCharacterizing cell adhesion by using micropipette aspiration.
We have developed a technique to directly quantify cell-substrate adhesion force using micropipette aspiration. The micropipette is positioned perpendicular to the surface of an adherent cell and a constant-rate aspiration pressure is applied. Since the micropipette diameter and the aspiration pressure are our control parameters, we have direct knowledge of the aspiration force, whereas the cel...
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ژورنال
عنوان ژورنال: Annals of Biomedical Engineering
سال: 2013
ISSN: 0090-6964,1573-9686
DOI: 10.1007/s10439-013-0791-9